Enzyme bio lab quizlet

A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence. More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. Start studying Bio 103 Lab Final. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 7.Which of the following are true regarding restriction enzyme a) restriction enzyme are used to cut DNA molecule b) restriction enzyme are used to construct restriction maps c) restriction enzyme are used in RFLP d) all of these 8. The type of restriction enzymes used in rDNA technology is a) Type I b) Type II c) Type III d) all of these 9. Virtual Lab> Journal Questions Yes it is a linear relationship because if initially as you add more substrate more products will be made but if you add too much substrate initially it won't show more products because maybe there's not a lot of enzymes to bond with,because all of them are already filled. 2.5.U1 Enzymes have an active site to which specific substrates bind. State the relationship between enzyme substrate and enzyme active site. Explain the relationship between enzyme structure and enzyme specificity, including the role of the active site. Nov 17, 2007 · we are doing a lab in biology called the penny lab. In this lab, we did 3 trials where one person would pick up the penny with one hand and flip it over so that it would be face up, the next person would count the pennies, and the last would write down the results. then we did the next set of trials where our hand was taped so it was very hard to pick up the pennies, and then we did the next ... The initial rate of an enzyme catalyzed reaction is always highest, due to the abundance of substrate molecules. But as time passes, fewer and fewer substrate molecules are availabe for reaction with the enzyme, and thus the rate of the reaction goes down. Function of Rennin Enzyme. Rennin is an enzyme that is essential for the digestion of proteins. It helps digest milk in young mammals. This BiologyWise article lists out the function of rennin enzyme. Exercise 3: The Peripheral Nervous System and Reflex Arcs Nov 17, 2007 · we are doing a lab in biology called the penny lab. In this lab, we did 3 trials where one person would pick up the penny with one hand and flip it over so that it would be face up, the next person would count the pennies, and the last would write down the results. then we did the next set of trials where our hand was taped so it was very hard to pick up the pennies, and then we did the next ... 048 - EnzymesPaul Andersen explains how enzymes are used to break down substrates. The correct shape of the active site allows a key/lock fit between the en... AP Biology Enzyme Lab Questions Pre-Lab Questions 1. In this lab you will be testing the factors that influence enzyme activity. What is the substrate you will be working with? 2. What is the enzyme you will be working with? From which organism will it be obtained? 3. What will be the byproducts of the observed reaction? 4.UMUC Biology 102/103 Lab 4: Enzymes Answer Key. This contains 100% correct material for UMUC Biology 102/103 LAB04. However, this is an Answer Key, which means, you should put it in your own words. Here is a sample for the Pre lab questions answered: Lab 4: Enzymes. ANSWER KEY. Pre-Lab Questions. 1. Molecular Biology-In this laboratory you will use some basic tools of molecular biology to gain an understanding of some of the principles and techniques of genetic engineering. In the first part of the lab, you will use antibiotic-resistance plasmids to transform Escherichia coli. In the second part, you will use gel electrophoresis to separate Paul Andersen explains the two major portions of the molecular biology lab in AP Biology. He starts by discussing the process of transformation. He explains how you can use the pGLO plasmid to produce glowing E. coli bacteria. He then describes how you can use restriction enzymes and the process of gel electrophoresis to cut and separate DNA. The enzyme catalase is present in most cytochrome-containing aerobic and facultative anaerobic bacteria. Catalase is the enzyme which has one of the highest turnover numbers compared to all other enzymes; one molecule of catalase has the ability to convert millions of molecules of hydrogen peroxide to water and oxygen in each second. Start studying Bio- Enzymes. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Lab 4: Enzymes. ANSWER KEY Pre-Lab Questions 1. How could you test to see if an enzyme was completely saturated during an experiment? (2 pts) To test this, youd simply see if the reaction was still taking place. If the enzyme was completely depleted, the reaction could no longer occur. 2. List three conditions that would alter the activity of ... Sep 25, 2012 · Lactase is an enzyme that breaks down the substrate of lactose. The lactose is broken down into glucose and galactose. Once its broken down its easy to use a glucose test strip to see how much glucose is in the lactose to see how much the enzyme broke down the substrate. Enzymes work within a small pH range, therefore pH levels tend to have a great impact on the enzyme-substrate activity (Nelson Biology 12). Any pH level above or below 7 started to denature the enzyme, slowing down the rate of reaction. Denaturing enzymes meant that the shape of the overall enzyme had changed.
What can our measurements tell us? We can plot our results to help us easily identify the factors that can change enzyme activity. There is is a clear link here between the practical and theoretical elements of biology as the impact of concentration (of enzyme and substrate), inhibition, temperature and pH all have characteristic effects on the rate of reaction plot.

This and salt concentration also affect enzymatic structures. As a result, enzymes denature at extremes of pH and high salt concentrations. In addition, substrates and/or the enzyme's active site groups may become ionized. This further affects the enzyme-substrate binding. Again the pH at which an enzyme works best is called its optimal pH.

Oct 24, 2009 · Fly lab report p. 7 Fly lab report p. Fig. 1. Taste response curves of flies to different concentrations of the sugars glucose, maltose, and sucrose. Fig. 2. Chemical formulas of sucrose and maltose (Biology Department, 2000). Glucose is a monosaccharide and is shown as part of each of these molecules.

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Today in biology, we did a lab where we had to find the product of different amounts of substrate in liquids with various phs. I learned that the ph affects the way the enzymes functions. I also learned the different ways an enzyme's function can be affected such as when there is a low substrate concentration, the initial reaction rate of the ...

Start studying Bio 103 Lab Final. Learn vocabulary, terms, and more with flashcards, games, and other study tools.

The interaction between the substrate and the enzyme takes place in a particular region of the enzyme mole­cule called the active site. In many in­stances compounds other than the normal substrate for a particular enzyme-catalyzed reaction may bind to the enzyme’s active site, and this has a significant effect on the kinetics of the normal reaction.

Sep 25, 2012 · Lactase is an enzyme that breaks down the substrate of lactose. The lactose is broken down into glucose and galactose. Once its broken down its easy to use a glucose test strip to see how much glucose is in the lactose to see how much the enzyme broke down the substrate.

AP Central for Education Professionals | College Board Electrophoresis is one of the most important techniques used by molecular biologists. To name only a few applications, deoxyribonucleic acid (DNA) electrophoresis is used to map the order of restriction fragments within chromosomes , to analyze DNA variation within a population by restriction fragment length polymorphisms (RFLPs), and to determine the nucleotide sequence of a piece of DNA. Nov 12, 2018 - The Amoeba Sisters GIFs (animations) are a collection we created to supplement our videos. GIFs are popular on social media, and they are AWESOME in science for animating vocabulary or scientific processes! Oct 25, 2009 · Liver tissue contains very high concentrations of the enzyme catalase (which is an enzyme that breaks down hydrogen peroxide ) as it is a high metabolising tissue. Mammalian catalase has an optimum (best) operating temperature of 37 degrees C. Enzymes have an optimum temperature at which they work fastest. For mammalian enzymes this is about 40°C, but there are enzymes that work best at very different temperatures, e.g. enzymes from the arctic snow flea work at -10°C, and enzymes from thermophilic bacteria work at 90°C. Up to the optimum temperature the rate increases